• The PyloriTek® test is a rapid urease test based on patented dry reagent technology.
  
• The PyloriTek® test detects urease which is produced in large quantities by Helicobacter pylori.
• Urea in the PyloriTek® substrate pad reacts with urease in an H. pylori positive biopsy to create ammonia gas.
• Ammonia gas passes through the semi-porous membrane and causes a dark blue or purple color to appear on the yellow pH paper directly above an H. pylori-infected biopsy.

1. Preparation of Test Strip

• Once you have determined that a rapid urease test is required, remove a test strip from the bottle, lay it down on a clean, dry surface.
• Write the patient name or identification number and the time the test is started on the lines provided on the front of the test strip.
• Turn the strip over and crease at the perforation.
• Add four drops of Hydration Reagent to the Substrate Pad (one drop in each corner of the absorbent pad at the end of the strip).

2. Apply Specimen

Using a clean applicator stick, plastic pick or any other suitable accessory, push the gastric specimen from the forceps onto the Reaction Pad. The gastric specimen should be placed at least 6-7 mm from the red positive control spot which is visible on the Reaction Pad. The gastric specimens should be placed on the raised area of the Reaction Pad which is on the opposite side from the yellow pH membrane. At the doctor's option, one, two, or three gastric specimens from the same patient can be placed on the pad. Keep the specimens at least 6-7 mm apart to ensure that each reaction can be viewed separately.

3. Fold and Insert Test Strip into Chamber

When your gastric specimens are in place, immediately fold the Substrate Pad on top of the Reaction Pad and insert the folded strip into the Reaction Chamber. Be sure the yellow side of the Reaction Pad is visible.

4. Interpret Results

Within 60 minutes, examine the yellow Reaction Pad for the appearance of intense dark blue or purple color spots. The purple positive control should appear in the upper right hand corner. The positive control spot usually appears within 4-10 minutes but may occasionally take longer. Most positive patient specimens appear in less than 20 minutes. Some very strong positives may appear in 1-2 minutes. A positive result may appear anytime up to the full 60 minutes. For a confirmed positive, both the specimen and the positive control have to show the strong blue/purple color. Always wait the full 60 minutes before listing a patient's result as negative.

The PyloriTek® Quick Reference Chart shows typical positive and negative reactions. This chart is available free of charge from your exclusive PyloriTek® distributor.